Automated Purification of Nucleic Acids ...

Automated Purification of Nucleic Acids | QIAcube HT

Supplier: QIagen Chadstone

Instrument for automated mid- to high-throughput nucleic acid purification in 96-well format. QIAcube HT enables automated mid- to high-throughput nucleic acid purification in 96-well format using silica membrane technology.

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A variety of QIAcube HT Purification Kits lets users quickly and easily purify DNA, RNA and miRNA from almost any type of sample – including cells, tissues and stool samples, as well as from bacteria and viruses in animal samples. Automated protocols and dedicated accessories and consumables increase reliability and convenience and save valuable time. The system provides the same high-quality results as other trusted QIAGEN purification technologies.

QIAcube HT Features:

Reliable DNA, RNA and miRNA extraction from virtually any sample type
Mid- to high-throughput solution with dedicated purification kits saves costs and time
User-friendly software allows for easy data management and documentation
Innovative design features increase safety and minimize cross-contamination
Convenient, flexible and easy-to-use instrument with small footprint

QIAcube HT Procedure:

QIAcube HT provides automated, high-throughput nucleic acid purification for 24–96 samples per run, in increments of 8 samples. The instrument uses a vacuum-driven system to perform the simple and fast bind–wash–elute procedure during sample purification. The vacuum chamber is composed of two separate compartments. This configuration is one of many measures that minimizes sample cross-contamination during purification.
All materials needed for sample purification are placed onto the instrument worktable prior to the run. A 96-well sample block contains the starting sample material that will be lysed and transferred to the filter plate during the run. Easy-to-fill disposable troughs contain all necessary buffers and reagents for sample binding, washing, and elution. Pipet tips are positioned on the right side of the worktable.
Liquid-handling tasks are performed by the 8-channel pipetting head, which provides fast transfer of liquids during the run. The software tracks tip use and internally updates tip availability as the run proceeds. QIAcube HT optimizes the use of plasticware with a smart and economical tip reuse strategy. After washing, the pipetting head moves the 96-well filter plate to the elution compartment, where purified nucleic acids are eluted into the elution microtubes. The included 8-well strip caps securely close the elution microtubes for sample storage.

QIAcube HT Software

QIAcube HT Software provides a user-friendly interface for operating the QIAcube HT instrument. The software wizard simulates the instrument worktable and makes it easy to set up and start a run with just a few mouse clicks. The software automatically calculates reagent volumes according to the number of samples, and fully documents every run. The software also allows you to import bar code databases, manage user access, and integrate Laboratory Information Management Systems (LIMS).

QIAcube HT Specifications

Features	Specifications
Accessories (supplied)	Vacuum Control Station, Vacuum Chamber, Pinch Valve, Channeling Adapter (Channeling Block and Channeling Block Holder), Transfer Carriage, Riser Block EMTR, Adapter Reagent Plate SBS, Adapter Reagent Plate R1, Tip Disposal Box Holder, Tip Chute, Tip Chute Protective Cap, Waste Bottle with Lid Assembly
Communications	RS232 and USB
Consumables (supplied)	QIAcube HT Plasticware (5 S-Blocks, 5 Elution Microtubes RS (EMTR), 120 x 8-Well Strip Caps for EMTR, 9 x 96 Filter-Tips OnCor C, TapePad), 3x Tip Disposal Box with Lid, 2x Reagent Trough Lid 170 ml, 3x Reagent Trough with Lid 70 ml
Dimensions	Instrument width: 540 mm (21 in.), height: 450 mm (18 in.), depth: 630 mm (25 in.); vacuum control station width: 495 mm (19 in.), height: 520 mm (20 in.), depth: 400 mm (16 in.)
Electrical	100–240 V AC, 250 VA, 50–60 Hz, overvoltage category II; mains supply voltage fluctuations are not to exceed 10% of the nominal supply voltages
HEPA filter	Preinstalled dual-cartridge 3M Air-Mate HEPA filter system provides positive pressure clean air within the closed hood.
Humidity	Operating humidity 40-70%
Notebook (supplied)	QIAcube HT comes with a notebook computer for added flexibility.
Pipetting system	8 pipetting channels, pipetting volume (20-200 µl)
Place of operation	For indoor use only
Pollution level	2
Precision	20-200 µl, less than 4% coefficient of variation
Software	Supplied with unlimited user license with free upgrades by web download
Table capacity	4 x SBS positions (127.76 x 85.48 mm), compatible with 96-well plates or tip racks 1 custom adapter for a reagent trough plate compatible to accept reagent troughs vacuum chamber with chambers for waste and elution
Throughput	24-96 samples in increments of 8 samples
Tip disposal	All used tips ejected externally; no waste accumulation on the worktable
UV lamp	Preinstalled UV lamp for worktable decontamination
Warranty	1-3 years warranty, depending on service package
Weight	QIAcube HT instrument: 41 kg (90 lb.); vacuum control station: 19 kg (42 lb.)

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QIAcube HT Features:

Reliable DNA, RNA and miRNA extraction from virtually any sample type
Mid- to high-throughput solution with dedicated purification kits saves costs and time
User-friendly software allows for easy data management and documentation
Innovative design features increase safety and minimize cross-contamination
Convenient, flexible and easy-to-use instrument with small footprint

QIAcube HT Procedure:

QIAcube HT provides automated, high-throughput nucleic acid purification for 24–96 samples per run, in increments of 8 samples. The instrument uses a vacuum-driven system to perform the simple and fast bind–wash–elute procedure during sample purification. The vacuum chamber is composed of two separate compartments. This configuration is one of many measures that minimizes sample cross-contamination during purification.
All materials needed for sample purification are placed onto the instrument worktable prior to the run. A 96-well sample block contains the starting sample material that will be lysed and transferred to the filter plate during the run. Easy-to-fill disposable troughs contain all necessary buffers and reagents for sample binding, washing, and elution. Pipet tips are positioned on the right side of the worktable.
Liquid-handling tasks are performed by the 8-channel pipetting head, which provides fast transfer of liquids during the run. The software tracks tip use and internally updates tip availability as the run proceeds. QIAcube HT optimizes the use of plasticware with a smart and economical tip reuse strategy. After washing, the pipetting head moves the 96-well filter plate to the elution compartment, where purified nucleic acids are eluted into the elution microtubes. The included 8-well strip caps securely close the elution microtubes for sample storage.

QIAcube HT Software

QIAcube HT Software provides a user-friendly interface for operating the QIAcube HT instrument. The software wizard simulates the instrument worktable and makes it easy to set up and start a run with just a few mouse clicks. The software automatically calculates reagent volumes according to the number of samples, and fully documents every run. The software also allows you to import bar code databases, manage user access, and integrate Laboratory Information Management Systems (LIMS).

QIAcube HT Specifications

Features	Specifications
Accessories (supplied)	Vacuum Control Station, Vacuum Chamber, Pinch Valve, Channeling Adapter (Channeling Block and Channeling Block Holder), Transfer Carriage, Riser Block EMTR, Adapter Reagent Plate SBS, Adapter Reagent Plate R1, Tip Disposal Box Holder, Tip Chute, Tip Chute Protective Cap, Waste Bottle with Lid Assembly
Communications	RS232 and USB
Consumables (supplied)	QIAcube HT Plasticware (5 S-Blocks, 5 Elution Microtubes RS (EMTR), 120 x 8-Well Strip Caps for EMTR, 9 x 96 Filter-Tips OnCor C, TapePad), 3x Tip Disposal Box with Lid, 2x Reagent Trough Lid 170 ml, 3x Reagent Trough with Lid 70 ml
Dimensions	Instrument width: 540 mm (21 in.), height: 450 mm (18 in.), depth: 630 mm (25 in.); vacuum control station width: 495 mm (19 in.), height: 520 mm (20 in.), depth: 400 mm (16 in.)
Electrical	100–240 V AC, 250 VA, 50–60 Hz, overvoltage category II; mains supply voltage fluctuations are not to exceed 10% of the nominal supply voltages
HEPA filter	Preinstalled dual-cartridge 3M Air-Mate HEPA filter system provides positive pressure clean air within the closed hood.
Humidity	Operating humidity 40-70%
Notebook (supplied)	QIAcube HT comes with a notebook computer for added flexibility.
Pipetting system	8 pipetting channels, pipetting volume (20-200 µl)
Place of operation	For indoor use only
Pollution level	2
Precision	20-200 µl, less than 4% coefficient of variation
Software	Supplied with unlimited user license with free upgrades by web download
Table capacity	4 x SBS positions (127.76 x 85.48 mm), compatible with 96-well plates or tip racks 1 custom adapter for a reagent trough plate compatible to accept reagent troughs vacuum chamber with chambers for waste and elution
Throughput	24-96 samples in increments of 8 samples
Tip disposal	All used tips ejected externally; no waste accumulation on the worktable
UV lamp	Preinstalled UV lamp for worktable decontamination
Warranty	1-3 years warranty, depending on service package
Weight	QIAcube HT instrument: 41 kg (90 lb.); vacuum control station: 19 kg (42 lb.)