PAXgene Laboratory Blood RNA Kit

PAXgene - Laboratory Blood RNA Kit

Supplier: QIagen Chadstone

Laboratory Blood RNA Kit

For high-throughput purification of cellular RNA from whole blood. Parallel purification of RNA from 96 samples, Standardized sample processing prior to analysis, No organic extraction or precipitation, Isolation of high-quality cellular RNA.

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The PAXgene 96 Blood RNA Kit is for high-throughput purification of cellular RNA in a manual format. Used with PAXgene Blood RNA Tubes (available from BD, cat. no. 762165) for sample collection and stabilization, the system provides a complete preanalytical solution for collection and stabilization through to purification of high-quality RNA to support high-throughput projects, such as clinical research studies.

Stabilization of gene expression.
- Blood was collected and RNA was isolated using the PAXgene Blood RNA System (for RNA stabilization and isolation). The graph shows changes in expression of 12 genes after blood collection, measured using real-time RT-PCR. (Data kindly provided by Source Precision Medicine, Boulder, Colorado, USA.)
Detection of tyrosinase following blood storage.
- Blood samples were collected from 6 different donors, spiked with tumor cells expressing tyrosinase, and stored for up to 10 days at room temperature. RT-PCR was used to measure levels of tyrosinase. [A] 1 cell per 2.5 ml blood; [B] 10 cells per 2.5 ml blood. WB: tyrosinase detection in RNA isolated from whole blood collected and stored in standard EDTA tubes. PAX: tyrosinase detection using the PAXgene Blood RNA System for sample collection, and RNA stabilization and isolation. (Data kindly provided by LMO, Munich, Germany.)
PAXgene 96 Blood RNA procedure.
- RNA isolation begins with a centrifugation step to pellet nucleic acids in the PAXgene Blood RNA Tube. The pellet is washed, and proteinase K is added to digest proteins. Alcohol is added to adjust binding conditions. Lysates are applied to the PAXgene 96 Filter Plate and centrifuged to remove cell debris. The lysate is then applied to a PAXgene 96 RNA Plate. Residual DNA is removed through a DNase I digestion. Remaining contaminants are removed in three efficient wash steps before pure RNA is eluted.
RNA isolation from blood using standard methods.
- Blood was collected and RNA was isolated using standard methods (collection in EDTA tubes; no stabilization; RNA isolation using a guanidinium-based method). The graph shows changes in expression of 12 genes after blood collection, measured using real-time RT-PCR. (Data kindly provided by Source Precision Medicine, Boulder, Colorado, USA.)

Applications

The RNA isolated from human whole blood is ready for use in a wide range of downstream applications, including:

Nucleic acid amplification technologies, such as RT-PCR and NASBA
Expression-array and expression-chip analysis
Quantitative RT-PCR, including TaqMan technology
cDNA synthesis
RNase and S1 nuclease protection
Northern, dot, and slot blot analysis
Primer extension

Specifications

Features	Specifications
Applications	PCR, real-time PCR, qPCR, NASBA, cDNA synthesis
CE/FDA/IVD compatible	Research use only
Elution volume	45–60 µl
Format	96-well plate
Main sample type	Whole blood
Processing	Manual (centrifugation or vacuum)
Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein	Cellular RNA
Sample amount	2.5 ml
Stabilization	Yes
Technology	Silica technology
Time per run or per prep
Yield	4–20 µg

To contact QIagen Chadstone about PAXgene Laboratory Blood RNA Kit use Get a quote.

Stabilization of gene expression.
- Blood was collected and RNA was isolated using the PAXgene Blood RNA System (for RNA stabilization and isolation). The graph shows changes in expression of 12 genes after blood collection, measured using real-time RT-PCR. (Data kindly provided by Source Precision Medicine, Boulder, Colorado, USA.)
Detection of tyrosinase following blood storage.
- Blood samples were collected from 6 different donors, spiked with tumor cells expressing tyrosinase, and stored for up to 10 days at room temperature. RT-PCR was used to measure levels of tyrosinase. [A] 1 cell per 2.5 ml blood; [B] 10 cells per 2.5 ml blood. WB: tyrosinase detection in RNA isolated from whole blood collected and stored in standard EDTA tubes. PAX: tyrosinase detection using the PAXgene Blood RNA System for sample collection, and RNA stabilization and isolation. (Data kindly provided by LMO, Munich, Germany.)
PAXgene 96 Blood RNA procedure.
- RNA isolation begins with a centrifugation step to pellet nucleic acids in the PAXgene Blood RNA Tube. The pellet is washed, and proteinase K is added to digest proteins. Alcohol is added to adjust binding conditions. Lysates are applied to the PAXgene 96 Filter Plate and centrifuged to remove cell debris. The lysate is then applied to a PAXgene 96 RNA Plate. Residual DNA is removed through a DNase I digestion. Remaining contaminants are removed in three efficient wash steps before pure RNA is eluted.
RNA isolation from blood using standard methods.
- Blood was collected and RNA was isolated using standard methods (collection in EDTA tubes; no stabilization; RNA isolation using a guanidinium-based method). The graph shows changes in expression of 12 genes after blood collection, measured using real-time RT-PCR. (Data kindly provided by Source Precision Medicine, Boulder, Colorado, USA.)

Applications

The RNA isolated from human whole blood is ready for use in a wide range of downstream applications, including:

Nucleic acid amplification technologies, such as RT-PCR and NASBA
Expression-array and expression-chip analysis
Quantitative RT-PCR, including TaqMan technology
cDNA synthesis
RNase and S1 nuclease protection
Northern, dot, and slot blot analysis
Primer extension

Specifications

Features	Specifications
Applications	PCR, real-time PCR, qPCR, NASBA, cDNA synthesis
CE/FDA/IVD compatible	Research use only
Elution volume	45–60 µl
Format	96-well plate
Main sample type	Whole blood
Processing	Manual (centrifugation or vacuum)
Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein	Cellular RNA
Sample amount	2.5 ml
Stabilization	Yes
Technology	Silica technology
Time per run or per prep
Yield	4–20 µg